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Sequencing errors should be considered a possibility and the anomalous sequences verified where appropriate.
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Reported melanoma in relatives was verified where possible [ 19].
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All yeast transformations were performed by the lithium acetate method and transformants were selected on the appropriate media and verified by PCR, and where appropriate, gene expression.
The results were verified by direct DNA sequencing where appropriate, using Eurofins MWG Operon's DNA sequencing service.
The patient's health passport, TB treatment card/ sheet and TB registers were evaluated where appropriate to verify the responses.
*Maternity and paternity leave verified with the company or, as noted where appropriate, courtesy of employee reporting on FairyGodboss.
Where appropriate, annotations were further refined, verified or adjusted using information retrieved from alternative databases, e.g. Uniprot/EMBL (http://www.uniprot.org/), protein family (Pfam) (http://pfam.sanger.ac.uk) and COGs [ 46].
A variable degree of right asymmetry (skewness from 0.5 to 1.6) of the parameter distribution was noted; where appropriate, statistical significance of the findings was verified, using log-transformed data.
ANOVA assumptions were verified preceding all statistical analyses; logarithmic transformations and Greenhouse-Geisser corrections were applied where appropriate.
Appropriate PCRs were used to verify the presence of the correct mutations in kanamycin-resistant transformants and, where appropriate, the loss of TpsA or TpsB expression was confirmed on Western blots.
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