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To test this hypothesis, we overexpressed GFP-tagged DAT-1(P dat-1 ::GFP DAT-1) in vt21 and vt22 and tested for Swip in lines verified to express GFP DAT-1 neurons.
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Using flow cytometry and confocal microscopy, the T47D and ZR-75-1 celinesnes were verified to highly express uMUC1, however it was found that only ZR-75-1 cexpressedessed the E-selectin binding moiety sialyl Lewis x (sLex).
LncRNA-ATB is verified to be aberrantly expressed in many cancers and promote the invasion-metastasis and proliferation cascades.
Transcripts that were verified to be differentially expressed in semimembranosus were subsequently tested in longissimus dorsi and supraspinatus muscle samples.
Seven of the transcripts were verified to be differentially expressed in the longissimus dorsi and semimembranosus by quantitative PCR with the exception being RPS6KA3, where differential expression was only verified in the longissimus dorsi.
MUC4 was verified to be highly expressed in PDAC and reversely related to prognosis [ 36, 37].
Of the 33 C4-2 genes PLD1, ACAS2L, CA1, CA9, ITM2A, CARD14, and GPR54 were verified to be uniquely expressed.
A leafy spurge SAND family gene was used as a reference; this gene was verified to be stably expressed during seed and bud development [ 38].
Nineteen genes exhibiting different expression patterns were validated by qRT PCR (Additional file 5: Table S4) and 10 house keeping genes [ 33] were verified to be stably expressed between the different treatments.
These selected genes were reanalyzed on TLDA or using individual qRT-PCR assays (n = 7 mice/group), and 112 genes were verified to be significantly differentially expressed (q<5%) in GF compared to SPF livers (Table S1).
These methods have been verified to be efficient in identification, expressed biodiversity in taxonomic system and unique in the selection of fungal strains.
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