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We further verified the specificity of the antibody in CEM cells [10], [13], [14].
We verified the specificity of the antibodies in western blot analyses (Figures 3A).
We verified the specificity of the signal by addition of ortho-phenantroline that suppresses fluorescent signal.
We verified the specificity of PCR by measuring the melting curve of the PCR product at the end of reaction.
As a negative control, the contralateral (or uninjured) side of the calvaria was imaged, which verified the specificity of GFP immunostaining (Figure 5A, upper right).
However, to avoid cross amplification we first cloned each of the PtSIT genes, and then verified the specificity of the primers used to amplify only a single SIT gene (see Materials and Methods).
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The melting curve was used to verify the specificity of the real-time qPCR reaction.
PCR products were sequenced to verify the specificity of the reaction.
To verify the specificity of the developed method, standards and samples were analyzed simultaneously.
Amplification products were visualized on 1% agarose gels to verify the specificity of probe-template binding.
Melting curves were analyzed at the end of the reactions to verify the specificity of each amplification.
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