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All PCR products were initially subcloned into TOPO-Blunt (Invitrogen) and their sequences were verified (The Sequencing Service, University of Dundee, UK, www.dnaseq.co.uk) before cloning into the transfection plasmid pHH1 or entry vector pHGB.
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We verified the sequence position of phosphosites in UniProt sequences using data from PhosphoSitePlus51 (see Supplementary information).
We then verified the sequence quality and filtered out ribosomal RNA reads and viral reads.
MPL verified the sequence analysis realized with IMGT, the server she conceived and maintains, and participated in the manuscript redaction.
32 In this investigation, we have verified the sequence-based prediction of vicinal cysteine with structural homology modeling.
Comparisons against selected type sequences in GenBank verified the sequences selected for RefSeq at type specimen level making sure the best sequence for the specimen was selected.
Technical replicates were included in order to verify the sequencing reproducibility (84 samples in total).
All the plasmids were sequenced to verify the sequence.
Tandem TOF was successful for identifying peptides up to m/z 5500 with no prior knowledge of the sequence and was also used to verify the sequence assignments for larger ion signals.
The PCR products were cloned and several independent clones were sequenced to verify the sequences.
The DNA was sequenced using both forward and reverse primers to verify the sequences.
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