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[22] We first verified the levels of PIAS3 in CAPN3 deficient muscle sections (Figure 2D).
Next, we verified the levels of cytokines in liver.
As an endogenous control for serum samples, we verified the levels of RNU6B and hsa-let-7a.
To ensure that the existence of the different 5'-leader sequences in dicer mRNA as revealed by the 5'-RACE analysis was not a PCR artifact, and also to determine relative quantities of each of the mRNA isoforms, we verified the levels of each of the isoforms by S1-nuclease protection assays (Fig. 4).
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We had previously verified the level of PRC expression during the kinetics of inhibition by comparing PRC expression in SiRNA to a negative control by quantitative RT-PCR.
Given that α6β4 integrin is the receptor for laminin 5 (LM5) and, as we previously demonstrated, ligation of the integrin to this substrate enhances PI3K signaling, we first verified the level of Akt phosphorylation upon stimulation in the mammary tumor cell lines.
Numerous studies have verified the level of IL-1β and TNF-α in human colon cancer [ 20- 23].
Independent measures of quality verified the level of quality in each facility; providing evidence that the RAI-MDS QIs were associated with the observed levels of care quality.
In this study we have verified the level of arsenic resistance and we investigated the presence of cytoplasmic arsenate reductase genes (arsC) in the genomes of the isolated strains.
Real-time PCR analysis was also conducted to verify the levels of SSN8 transcript.
Next, we isolated total RNA from the recombinant BCG and the parent strain to verify the levels of expression of ldtMt2.
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