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By regarding also the identified conserved mature miRNAs not covered by miRDeep and the known porcine sequences deposited in miRBase, we have verified the expression of 332 miRNAs expressed in the porcine intestine including 201 apparently novel porcine miRNAs (194 miRDeep predicted plus seven conserved mature miRNAs) corresponding to 61%.
We verified the expression of YER034W, PRM5 and FMP21 in NFRI 3236, NFRI 3155 and S288C under heat stress conditions.
qPCR analysis verified the expression of all eight transcription factors (8TFs) in HFF transduced with this new cocktail for three days (Fig. 3C).
To confirm the targeting ability of the immuno GNCs, we first verified the expression of CD44 receptors on 4T1 cells by immunohistochemistry and then quantified the number of GNCs taken up by 4T1 cells.
RT-PCR analysis verified the expression of the surface antigens at the mRNA level.
We verified the expression of both ecSOD and ecSOD-ΔHBD in total lysates (Fig. S4A).
We verified the expression of endogenous Ptc1 protein in this cell line with a commercially available antibody.
We identified several miRNA expression patterns, and verified the expression of selected miRNAs of each pattern with qRT-PCR.
Several in vitro studies verified the expression of VASP at endothelial cell-cell and cell-matrix contacs in different vascular beds [15] [19].
We verified the expression of several known and predicted targets of these adult and embryonic liver-enriched miRNAs in the liver tissue samples, using qRT-PCR.
In this work we verified the expression of the core clock genes cry1, cry2, clock, and cycle in the brooding coral F. fragum.
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