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We have verified (Supplementary Figure S3) that the heterogeneity is not due to the degree of DC differentiation, but other sources of cell-to-cell variability can of course exist.
M. arginini-specific PCR detection was verified (Supplementary Figure 1b).
These included regions curated from the literature as well as a small set of unpublished enhancer sequences with activity in the heart (supplementary material Fig. S1), which we had previously identified and empirically verified (supplementary material Table S1).
The efficacy of these markers of apoptotic induction was verified (Supplementary Figure S5D), using MII oocytes cultured in the presence of 200 nM DXR for 14 h (Bax, cytochrome c) or 20 h (pan-caspase).
Note that recombinant human HDACs, the purity of which was verified (supplementary Fig 1A online), might still be missing further crucial components or post-translational modifications relevant for their function.
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Real-time RT-PCR was performed to verify this (Supplementary Data, Supplementary Figure S2).
The close packing of the monolayer, and hence compatability with building well-defined interfaces, was verified electrochemically (Supplementary Fig. 1) by showing that redox species in solution could not access the underlying ITO surface and hence redox peaks were absent in the cyclic voltammetry.
As verified in Supplementary Figure S1 at (http://www.BiochemJ.org/bj/440/bj4400185add.htm), an inhibitor (Dynole 34-2) that lowered the percentage of nuclei in multinucleated Sf9Op1D cells also shifted the distribution of the number of nuclei per cell towards cells with lower number of nuclei.
We also found 58 NFRs lie in 58 distinct ORFs (25 verified ORFs, see Supplementary Table 13 in Supplementary Materials S1; 12 uncharacterized and 21 dubious ORFs, see Supplementary Table 14 in Supplementary Materials S1).
All structures were verified by NMR (Supplementary Figs 5–26).
The data from immunoblotting verified these results (Supplementary Figure 2).
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