Sentence examples for verified specificity from inspiring English sources

The qPCR enrichments obtained for Ramos ChIPs were independently verified by qualitative, traditional PCR amplification and ethidium bromide visualization (Fig. 3E) and westerns verified specificity for AID and monoubiquitinated histone antibodies (Fig. 3F).

Since the specificity of many antibodies against nAChRs was put in doubt [ 111, 112], we had verified specificity of our antibodies in the α7 and α3 knockout mice (data not shown).

Since there are too few experimentally verified specificity determining positions to perform a reliable analysis of observed column patterns, we instead use our EC Pfam dataset to address the question.

A melting curve was obtained to verify specificity of the PCR.

PCR products were analyzed by agarose gel electrophoresis to verify specificity of the products.

Isotype and secondary antibody controls were performed for each staining to verify specificity.

To verify specificity of pharmacological agents, we downregulated BK and IK1 channels in U251 cells using gene-specific siRNAs.

Melt curve analyses were performed for all SYBR Green PCR amplifications to verify specificity of the reaction.

Each sequence was designed to amplify a region nested within an amplicon produced from RT-PCR, and was screened using a BLAST inquiry to verify specificity.

Negative controls were included in which cells were incubated in the presence of secondary antibody only to verify specificity of staining.

To verify specificity, a parallel specimen was incubated under identical conditions, but in the presence of a 10-fold molar excess of the ALDH-inhibitor, diethylaminobenzaldehyde (DEAB).