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Snap-frozen primary tumour samples were collected from histologically verified SE (26), NS (24), combined tumours (CT) (6) and SSE (5) obtained from patients attending the Royal Marsden Hospital NHS Trust and Erasmus MC University Medical Center, Rotterdam (Supplementary Table 1).
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Annotations of tRNAs were completed with RNAWeasel and verified using tRNAscan-SE.
Protein-coding genes were also checked using the ab initio gene-finding program Glimmer v3.02 [ 30]. rRNA genes of the draft assembly were identified using RNAmmer [ 31]. tRNA genes for all 20 amino acids that were predicted by the RAST were further verified using tRNAscan-SE [ 32].
X-ray diffraction (XRD) analysis indicated that the films were single phase Bi2Se3 compound with orthorhombic structure, and the 2 3 stoichiometric ratio of Bi to Se was verified by EDX quantitative analysis.
On the basis of the successful synthesis of CdS nanocrystals, alloyed CdSxSe1−x nanocrystals can also be prepared by simply replacing certain amount of S precursor with equal amount of Se precursor, verified by TEM, XRD, EDX as well as UV Vis absorption analysis.
In addition, all tRNA genes were further verified by using tRNAscan-SE (http://lowelab.ucsc.edu/tRNAscan-SE/).
Successful grafting of pMPC from the SE surface was verified by attenuated total reflection-Fourier transform infrared spectroscopy and elemental analysis.
Therefore, it was verified that encapsulating Sal into SE-NPs can reduce its side effects.The possible reasons for this are that (a) SE-NPs can target Sal delivery to tumor tissue which can increase the tumor's drug concentration and decrease drug concentration of normal tissues and (b) sustained release can keep the normal tissues out of lethal threshold.
The ratio of Bi to Se is nearly 2 3, verified by energy-dispersive X-ray spectroscopy and with the addition of surfactant.
Sixty tRNA overlap genes that were preliminarily predicted by the RAST were further verified to be 30 using tRNAscan-SE.
By employing the mean difference of the PF, it was verified that TEs sweat more than SEs.
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