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Dr. Sinclair's new report, if verified, resolves this problem by showing that sirtuin has retained its genomic repair role in higher organisms but that the repair is focused on a different kind of genomic damage — that of breaks in a chromosome.
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The PCR product sizes were verified by resolving samples on agarose gels and PCR products were sequenced to confirm the identity of each target.
Each potential SNP and insertion/deletion was verified and resolved using the sequences from the cloned PCR product.
The integrity of RNA was verified by resolving in 1% formaldehyde-agarose gels and subsequent ethidium bromide staining.
Protein integrity was verified by resolving aliquots on a 4 12% NuPAGE Bis-Tris gel in MES buffer (Invitrogen).
First, we verified the resolving power of our SNP markers to discretely differentiate the two steelhead lineages that have been characterized in previous studies based on other genetic markers (e.g., allozymes, Currens et al. 2009; microsatellites, Blankenship et al. 2011), while confirming lineage of origin for each population.
Based on our long-standing policy restricting the sale of intangible items on eBay, we are discontinuing a small number of categories within the Metaphysical category, as transactions in these categories can be difficult to verify and resolve.
A backward translation was made to verify and resolve any semantic or conceptual problems.
In addition to the aforementioned process, two experts outside of the study team, as recommended in the literature, randomly selected sections of the transcripts and independently followed the aforementioned process to subsequently verify and resolve identified coding and thematic issues.
The different electrochemical properties of semiconductor electrodes modified with deposits from metal ion solutions compared to MNC modified electrodes are verified with time resolved potentiostatic measurements.
Themes were then checked, verified and differences resolved by discussion (Authors: TJ, LC, CJ).
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CEO of Professional Science Editing for Scientists @ prosciediting.com