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It is interesting, though, that choosing experimentally verified reaction rates we get quantitative agreement with experimental data as to where the transition in molecular composition is located.
Briefly, the modified algorithm gives preference to inclusion of verified reaction cascades (canonical pathways) into shortest paths by on-the-fly insertion of additional edges, which represent short-cuts within verified reaction cascades.
In addition, post-analytical melt curves from 55°C to 95°C were used to verify reaction quality.
Implementation of the proposed system provides a novel research tool, which is expected to contribute to the expansion of knowledge on free-radical reactions in monomer- and polymer solutions, by delivering precise kinetic data on changes in molecular weight and size, and thus allowing to formulate or verify reaction mechanisms.
A dilution series to verify reaction efficiency as well as no template controls were included in each run.
The formation of bonds of different types is verified: phosphite reactions with hydroxyl chain ends lead to the incorporation of significant quantities of phosphorus into the polyester backbone.
The specificity of PCR was verified by reactions without RT (–RT) and by melt curve analysis of PCR products.
The specificity of the RT-PCR was verified by reactions without RT (-RT) and by sequencing of the PCR product (University of Leeds, UK).
Specificity was verified by negative control reactions in which the primary antibody was replaced with normal rabbit serum.
The specificity of labeling by this procedure was verified by negative control reactions using buffer to replace the primary antibody and isotype-specific immunoglobulin G (IgG).
Certain observations from SA were verified by reaction mechanism, reactivity test and/or characterization techniques, while others need further investigation.
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