Total RNA was extracted from the tissues using TriReagent (Molecular Research Center) following the manufacturer's instructions, and verified for quantity and integrity by denaturing electrophoresis gel for RNA.
Total RNA obtained from head kidney tissue was verified for quantity and integrity by denaturing electrophoresis and labeling with Cy3- and Cy5-dCTP (Amersham Pharmacia) was completed using SuperScript III reverse transcriptase (Invitrogen) and oligo dT) primer, and cDNA was purified with Microcon YM30 (Millipore).
The illuminance value is an indicator for verifying the quantity of light in a given environment.
The SDS-PAGE gels were stained with Coomassie blue solution before the electrotransfer of proteins to verify the quantity of the protein recovered and the quality of separation.
Total RNA isolated for the microarray analysis was used to verify the quantity of specific messengers by real-time PCR for 4 biologically relevant differentially expressed genes (i.e., FGFR4, ESR1, ERBB2, FBLN2).
We tried to minimize potential recall errors by focusing our analyses on care-seeking; however, we were not able to verify the quantity of ANC visits reported by women in our sample or the content of the counselling they received during these visits.
Two plant samples treated with sterile water were further verified for the quantity of RNA using an ultraviolet spectrometer and electrophoresis on a denaturing formaldehyde agarose gel.
Also transmission of moments technique is applied on these models to verify the quantities that have been determined rigorously by MC technique.
Product size, quality and quantity were verified by agarose gel electrophoresis.
After one round of amplification of 300 800 ng of total RNA, RNA quality and quantity was verified (Agilent Technologies, Waldbronn, Germany).
