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We analysed pooled var gene transcription data from verified quantitative real time PCR measurements of a diverse set of clonal parasite cultures using a statistically rigorous method of parameter estimation.
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Aim: Perform a morphometric analysis of the myelinic fibers of the right hypoglossal nerve, in two age groups; to verify quantitative changes as a result of the aging process.
This verifies quantitative enrichment for rare alleles.
The quantitative competency of this approach was assessed by exploiting "limiting dilution assay" for absolute quantification, which provided an independent gold standard from which to verify quantitative accuracy.
Although this may not be a significant factor for highly characterized genomes, this approach can be effective for verifying quantitative accuracy for disparate species and/or new genotypes (see Results for additional details).
Differences in gene expression between the groups were verified using quantitative polymerase chain reaction [QPCR].
Single exon deletions in MLH1 and MSH2 can be verified using quantitative PCR.
Changes in gene expression were further verified by quantitative real-time RT-PCR.
This was also verified using quantitative PCR analyses (Fig. 1E).
Regulation of subsets of these genes was verified by quantitative PCR in an independent experiment.
Reduction of α-gustducin message was verified by quantitative real time PCR.
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