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Specifically, for each verified mature miRNA, we used a same-size sliding window and selected all possible sequences which could be created by sliding 1 base pair towards either direction from the verified mature miRNA over the precursor sequence, excluding any hairpin loops.
In this work we use only experimentally verified mature miRNAs and their corresponding precursors.
Overall, only experimentally verified mature miRNAs were used to form the positive class in both training and evaluation datasets.
Our method can accurately predict the start position of experimentally verified mature miRNAs for both human and mouse, achieving a significantly larger (often double) performance accuracy compared with two existing methods.
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To verify that mature adipocytes were labeled following H2BGFP pulse, we dissociated adipose tissue from pulsed Rosa26-rtTA; tetO-H2BGFP animals and stained individual H2BGFP-positive adipocytes for perilipin (Figure 1D).
Experimentally verified human and mouse mature miRNAs from the miRBase database (version 14) (http://www.mirbase.org) were used to train and evaluate our model.
The correct splicing of the White intron was verified by sequencing the mature mRNA transcripts of DsRed over-expression and down-regulation Drosophila transformed lines.
These results were confirmed and extended to later seed development stages by a RT-qPCR analysis on wild-type embryos at 13, 16 and 19 Days After Pollination (DAP), compared to mature seeds, to verify whether the DAG2 gene was expressed also during seed maturation.
At the same time, they succeeded in amplifying the pri-miRNAs, pre-miRNAs, and mature miRNAs from mature pollen cDNA, which verified that miRNA synthesis in pollen was going on as usual [ 38].
It was verified by the presence of mature bone in the peri-implant bone in the 9M specimens [4, 22, 43].
Thirty three novel miRNA precursors encoding 35 mature miRNAs are verified by PCR (Figure 5A).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com