Sentence examples for verified loss from inspiring English sources

Western blot analysis using anti-PfPV1 antisera verified loss of the circa 50kDa endogenous PfPV1 band, but the appearance of an approximately 77 kDa band, corresponding to the predicted molecular weight of the PV1-GFP chimera (Fig. 6A, right).

Despite ubiquitous expression of Ncu-g1 (Steffensen et al., 2007; Schieweck et al., 2009) and the verified loss of transcript in all assayed organs, this study suggested that lack of NCU-G1 predominantly affects the liver.

Colonies were screened using PCR primers for the gene sequence of the target gene to verify loss of the gene.

Initially, we wished to verify loss of the tk gene from the clonal parasites, as this would be characteristic of a double crossover integration event.

The following day, colonies were co-inoculated in LB with Ampicillin and Ampicillin + Kanamycin to verify loss of the Kan R prior to plasmid isolation.

Single colonies which grew on the control plate but did not grow on the selection plates were noted and antibiotic susceptibility testing was performed on them to verify loss of resistance.

To verify loss of DNA due to handling or equipment mishaps (i.e. clogged capillary or tip), each microtiter plate was cultured in duplicate and replicates were processed using the same instrument model but on different physical units where available.

Amplification of PCR fragments for transformation, to verify loss of CORE-GS, and to amplify genomic DNA for sequencing was as described previously [ 14] and utilized either Taq or exTaq (TaKaRa).

Hippocampal extracts from Fmr1 KO rats and controls (n = 3/age for developmental expression; n = 5/genotype at P10 to verify loss of expression) were prepared in RIPA buffer containing protease inhibitors (Complete EDTA-free), immunoblotted using primary antibody to FMRP (1 2000; AbCAM ab69815) and imaged as previously described in (13).

In this regard, we used a previously characterized L278Q loss-of-function mutant [22], [35] and verified the loss of catalytic activity by comprehensive analyses of GDP/GTP exchange in vitro and by in vivo assays for F-actin induction by the N-terminally truncated Vav (Fig. S4 and [9], [36]).

If this terrible news be verified, the loss of the Titanic will rank as the worst shipping disaster in the history of the world.