Sentence examples for verified in sequencing from inspiring English sources
Furthermore, specificity of the PCR reactions and the utilized primers for MMP analyses was verified in sequencing analyses.
The forward primer, 5'-CGC GGA TCC GAA ACC ATG AAC TTT CTG CTG TCT T-3' with a BamH1 restriction site, and the reverse primer, 5'-GCT CTA GAC CCG GCT CAC CGC CTC GGC TT-3' with an Xbal restriction site, were designed, and the PCR products of the VEGF isoforms from normal lung tissue mRNA were purified and verified in sequence, and were used to transfect competent cells.
Candidate GH1s were synthesized, cloned, and sequence verified in-house.
IMAGE cDNA clones representing 72 human genes involved in cell migration were purchased from Research Genetics (Huntsville, AL) and sequence verified in-house.
To further validate the significance of the RNA-seq-derived differential expression analysis, the constitutive expression of an array of housekeeping loci commonly used in qRT-PCR normalization [ 69– 75] was verified in the sequencing datasets after library size normalization.
To further validate the significance of the RNA-seq-derived differential expression analysis, the constitutive expression of an array of housekeeping loci commonly used in qRT-PCR normalization (Ferreira and Cronje 2012; Huggett et al. 2005; Langnaese et al. 2008; Silveira et al. 2009; Wan et al. 2011) was verified in the sequencing datasets after library size normalization.
All candidate mutations were verified in independent PCR and sequencing reactions and within one week interesting mutants could be selected and weaned.
These ambiguities could be verified in separate PCR and sequencing reactions, and they occurred predominantly at synonymous positions that were also variable between species.
The sequences of the amplicons were verified by sequencing in 3730 DNA Analyzer automated sequencer (Applied Biosystems).
Mutations were verified by sequencing in an ABI3100 Capillary DNA Sequencer.
Predicted boundaries of small and large ribosomal subunit RNA genes were verified in alignment with sequences from both Naegleria and jakobid mtDNAs.
