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Fresh tumor samples (verified from frozen sections), aseptically collected in the operating room, were minced with scissors in a petri dish.
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This verified that RNA from frozen tissue had the most optimal quality for subsequent microarray analysis.
All samples were verified as frozen on arrival at Doctor's Data.
Additional surgery was verified from medical records.
We isolated total RNA from frozen tissue and cells using the RNeasy kit (Qiagen) and verified RNA integrity with an Agilent Bioanalyser 2100 (with typical 28S/18S ratios = 2 ± 0.1).
Huge meteorite from dramatic Russian fireball pulled from frozen lake.
They risk their lives to rescue dogs from frozen lakes.
VR obtained RNAs from frozen berries.
DNA was extracted from frozen tissue using standard procedures.
RNA was extracted from frozen samples using TRI Reagent RT Molecular Research Centerr, Cincinnati, OH).
Cultures were plated and grown overnight from frozen stock.
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