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Herbarium records from 249 institutions were obtained and verified for correct identification.
Prior to in vitro expression, all products were verified for correct size and purity.
All peaks were verified for correct chromatographic peak selection and integration.
All samples used for RNA-sequencing were also strictly verified for correct identity (Additional file 1).
ES cell clones (192) were selected and verified for correct recombination using long-range PCR and Southern blot analysis and 1(c)).
If an in vivo implementation is intended, the cloned plasmids are verified for correct function in vitro before in vivo implementation.
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A PCR check for integration was performed using the following primers to verify for correct plasmid integration (1185 bp): pENO1 FW: 5′-TCCTTGGCTGGCACTGAACTCG-3′ and dTom REV: 5′-AAGGTCTACCTTCACCTTCACC-3′.
The inserts were verified for their correct annotation by DNA sequencing.
After extraction the plasmid was verified for the correct insert by sequencing and quantified as described (7 ).
The positive clones were sequence verified for insertion and correct orientation of the epitope tag.
The oxidised PrP fraction was lyophilised, and verified for purity and correct folding using SDS-PAGE and circular dichroism spectroscopy.
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CEO of Professional Science Editing for Scientists @ prosciediting.com