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Investigations have verified fish cell lines for bystander studies, including EPC (endothelial progenitor cell), RTG-2 (Oncorhynchus mykiss cell line; testis ovary mix), RT-Gill (Oncorhynchus mykiss cell line; gill) W1, and CHSE-214 (embryonic fish cell line) (O'Neill-Mehlenbacher et al. 2007).
Nevertheless, we verified FISH status in all patients with positive HER2 IHC expression in the brain lesions, and indeed, the proportion of FISH-positive cases among otherwise IHC HER2-positive cases seemed to be lower compared with respective figures in primary tumors.
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These ACTH challenge tests also revealed that the impairment of the cortisol response verified in fish exposed to atrazine + simazine and glyphosate, as shown in our previous work, seems to be related to steps of cortisol secretion in higher levels within the HPI axis.
Two mis-assembled scaffold were verified by FISH with newly developed single-copy genes.
Additionally, we constructed a linear regression model to verify whether fish consumption (summed across fish types) during the third trimester predicted bloodspot Hg concentration.
Identification of scaffolds as hybrid or misassembled by virtue of unequal hit coverage was verified by FISH-TSA.
Verify the fish is dead With a net, gently scoop up the fish.
To verify our FISH data and obtain higher resolution we used quantitative real-time reverse transcription PCR (RT-PCR) with several primer pairs encompassing nearly all of the non-repetitive, non-genic sequences across the entire β-globin locus.
This version contained 366 FISH verified BAC/PAC clones that span genomic regions implicated in over 43 known genomic disorders [32] as well as all 41 subtelomeric regions.
Subsequently, an expanded version of the array, CMA V5 that contains 853 FISH verified clones was implemented that detects gains and losses due to genomic rearrangements associated with over 70 disorders.
Pooled RNA of each fish family, previously used for microarray analysis, was used to verify a set of differentially expressed genes from the microarray experiment and to evaluate relative abundance of iron genes between infected and control fish.
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