Suggestions(1)
Exact(9)
Non-quantitative immunoblot analysis verified expression of F. novicida Hfq protein in the E. coli mutant strain GS081 (Fig. 2C).
We have verified expression and muscular dystrophy in the H-FRG1TG mouse and seen that thigh-derived myoblasts, but not diaphragm-derived myoblasts, from these animals demonstrate a proliferative defect by clonal analysis.
However, another study verified expression of genes inserted into integrons under the control of a hybrid Pc configuration (11 ).
Note that this does not control for false discovery rate and may overlook significant expression differences less than 2-fold, but we verified expression differences in the genes of primary interest using qPCR.
We have verified expression of the Il4i1_2 variant in mouse testis and brain, and thus show that the previously reported NUP62 promoter also drives expression of a second gene in a cell-type and tissue specific manner.
This formed a sequence verified expression cassette using the 35S promoter driving expression of a chimeric gene that encodes a C-terminal fusion of the GFP protein to the At2g34700 protein.
Similar(51)
Property (ii) is easily shown since (20) is negative, given that it verifies expression (14) and W > 0.
To verify expression of COL1A1; 2.0 µg of total RNA was reverse-transcribed using Superscript-III reverse transcriptase (Invitrogen Carlsbad, CA), according to manufacturer's protocol.
After successful incorporation of HIV genes we next sought to verify expression of our transgene and capsid incorporation at the protein level by Western blot analysis.
Quantitative PCR was used to verify expression of selected genes.
Gel electrophoresis was used to verify expression and the size of the product.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com