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Despite the lack of extensive information on experimentally verified essential genes, the little evidence that exists, supports our predicted essential roles for a number of genes: metK [ 49], guaA [ 50] and ntpA-B-C-D-E-F-K [ 51].
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As a first mutagenesis approach we verified the essential role of the two carboxylic acid residues E172 (putative proton donor) and D170 (putative stabilizer) in the catalytic mechanism of Chit42, and additionally the role of the carboxylic acid E145 (putative proton donor) in the catalytic mechanism of Chit33.
Previous reports have verified the essential roles of uPAR in tumor invasion and metastasis [ 29, 30].
We verified respondents' essential statements by contrasting them with our quantitative data, local documents and/or international and Dutch literature.
Knockdown of CDK8 verified its essential role for basal STAT1-S727 phosphorylatinNKin NK cells and significantly enhanced cytotoxicity.
We verified the essential nature of these motifs for rhombomere-specific activity of the enhancer through mutagenesis of the zebrafish element followed by reporter assay (Methods).
Tests during production and at operational conditions are performed to verify that essential requirements are met.
Hence, a cell verifies whether essential conditions are satisfied at checkpoints in G1, S, G2 and M phases, respectively.
The investigator must allow the monitor to verify all essential documents and must provide support at all times to the monitor.
Not long after Hicks' death in 1849, German pathologist Rudolf Virchow (1821 1902) coined the term zoonosis, verifying the essential link between animal and human health (5 ).
Consistently, the expression of tyrosinase and Tyrp1 was significantly suppressed at the mRNA or protein levels in cultured hyperpigmented skin in the presence of an ET-B antagonist, BQ788, even for 3 days (Fig. 3B-D), verifying the essential role of the ET-1/ET-B signaling pathway in the maintenance of hyperpigmentation.
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