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To quantify the degree of editing we verified editing of mir-377 by qRT-PCR analysis.
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At least 12 putative gene losses have been documented in Erodium[ 7], and mitochondrial genes sequenced from Pelargonium x hortorum had a drastic reduction in predicted or verified RNA editing sites compared to all other angiosperms examined [ 1].
The recorded message then goes through an editorial process, and is verified and edited by a set of trained journalists and volunteers to avoid any propaganda or false claims.
Any user-submitted content that it publishes via i3 will be verified and edited by Cleantech Group analysts, said the firm's chief executive officer Sheeraz Haji.
These transcripts were then verified and edited by the first and the third authors.
The alignment was manually verified and edited [see Additional file 1].
Names are removed from the data, and are verified and edited before entered into a computer file.
LTR regions were aligned using Clustal X [ 61] and verified and edited by eye using Se-Al ver 2.0 [ 62].
Suspicious genotypic scores identified from the genotype probabilities table calculated by JoinMap, were verified and edited when necessary.
This initial automated annotation was verified and edited manually by using criteria such as the presence of a ribosome-binding site, GC frame plot analysis and similarity to known protein-encoding sequences (CDS).
Sequences were verified and edited in CodonCode Aligner (CodonCode Corp .. Samples that exhibited two bands were cloned into pGEM-T Easy Vector System I (Promega), and plasmids were purified using the UltraCleanTM Plasmid Prep Kit (MO BIO Laboratories).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com