The overall mechanism of color change was verified to be corresponding to the different redox states of PANI regulated by Cu species during the photochromic process.
The in-house vector presented here was constructed by combining commercial pUC18 and the pDONR-221 plasmid's ccdB gene encoding region; toxic gene system sequence integrity was verified by sequencing, corresponding to the expected size (659 bp).
The highest xylanase activity was verified at 25 °C, corresponding to 51.43 U/mL.
Based on 2010 data, nominal tax revenue per pack is calculated at €2.78, a value verifying and corresponding to the fixed amount of tax (86% of the nominal retail price).
RNA quality was verified by observing bands corresponding to 28S, 18S and 5S ribosomal RNA on denaturing agarose gel and ensuring an approximate 2∶1 intensity ratio between 28S and 18S bands.
For the p15Ink4B probe we PCR amplified, subcloned, and sequence verified a cDNA fragment corresponding to nucleotides 70-450 (relatove thethe start of translation).
As a result, we have collected 42 experimentally verified YY1-binding motifs, corresponding to 21 target genes from human and 10 from mouse (Table 1 and Supplementary Tables S1 S2).
