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The absence of DNA template was verified by replacing the RT/Taq mix by the Taq DNA polymerase only.
The specificity of each assay was verified by replacing the primary antibody with unrelated mouse IgG in buffer at comparable dilution.
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Specificity of staining was verified by either replacing the primary antibody with normal serum or preabsorbing the antibody with its corresponding immunizing peptide (sc-52P for c- fos, Santa Cruz Biotechnology, at 1 μg peptide per 1 μl anti-serum; AG344 for NR1; AG305 for GluR2/3, Chemicon, USA, at 1 μg peptide per 5 μl anti-serum).
Then, topological features corresponding to the starting ideas and design step sequence are verified individually by replacing the related devices with ideal behavior model.
MasterCard and Visa have announced plans to replace their much disliked online security systems, MasterCard SecureCode and Verified by Visa, replacing them with an easier to use system.
The specificity of labeling by this procedure was verified by negative control reactions using buffer to replace the primary antibody and isotype-specific immunoglobulin G (IgG).
They are not verified by a medical professional and should not replace professional medical instruction.
The amplified fragments were cloned into the pBL-CAT2 plasmid upstream of the coding region of the CAT gene, replacing the thymidine kinase promoter, and verified by sequencing.
Correct allelic replacement was verified by PCR.
Specificity was verified by negative control reactions in which the primary antibody was replaced with normal rabbit serum.
Y397 was replaced by phenylalanine and the FAK mutation was verified by sequencing.
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