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The dwell time in these methods ranged between 100 and 175 ms. In each method, the possible cross-talk between transitions was verified by inserting a dummy [M + H]+ → product ion transition between those for [11C]species and [M + 1]carrier.
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First, common haplotypes (at least 1 5% frequency) of the TH promoter were generated by site-directed mutagenesis, then verified by sequencing, and inserted into luciferase reporter vectors.
Where promoter sequences were obtained, the promoter regions were PCR amplified, verified by sequencing and inserted as HindIII-SacI fragments the pGPTV-GUS-KAN [ 2, 53] or pGPTV-GFP-KAN binary vectors.
Cloned inserts were verified by PCR using insert specific primers and by restriction digestion using the EcoRI enzyme with restriction sites within the pCR4 vector.
The strong hairpins formed by the mirrored pairs prevented sequencing through them, so their successful insertion was verified by checking the insert length via PCR and by the presence of an un-sequenceable hairpin.
The desired sequence of the resulting plasmids was verified by sequencing the insert.
One implementation, which is verified by experiment, is to insert thermal resistant layer between adjacent batteries to prevent TR propagation in the battery module.
The final constructs were characterized by restriction analyses and verified by sequencing of the inserted DNA.
All recombinant vectors were verified by sequencing of the inserted fragments.
Their system will be distributed as part of a CD known as a "live CD," making it possible to compute or verify the hash just by inserting the disk in a computer.
Random ampicillin-resistant colonies were selected and grown in 5 ml LB-ampicillin broth, plasmids purified [ 63], restricted with NotI/XbaI and insert size verified by 1% agarose gel electrophoresis [ 63].
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