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The MEK1 nt 199 G→A transition was introduced using Quick-Change Site-Directed Mutagenesis (Stratagene, La Jolla, CA) and verified by direct sequencing.
The insertion of WL mutation was verified by direct sequencing in both the forward and reverse directions.
Finally, obtained sequences were verified by direct sequencing, using sequencing primers designed to cover target regions in both directions.
These were subsequently used for calculating the solid COD output, verified by direct COD measurements.
Nucleotide sequence variations in the different SSCP patterns were verified by direct DNA sequencing.
The predictions of this model are verified by direct numerical simulations using the beam propagation method.
This could be verified by direct measurements of the parameters of fluid-jet stimulation.
The results are verified by direct observations of the sawing channel of an experimental setup.
All analytical solutions have been verified by direct numerical integration of the modal equations of motion.
The validity of the stability diagram is verified by direct numerical integrations of the equations of motion of the system.
A particular solution instability is found to exist using Hsu's method and is verified by direct numerical integration.
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