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The melatonin was Microtonin™ 500 μg manufactured by Cardiovascular Research, Ltd., Concord, CA. The melatonin content of the preparation was informally verified by diluting capsules in water and assaying with the Bühlmann Melatonin Direct RIA.
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This was verified by serially diluting cDNA samples, performing qPCR with the different primer pairs and calculating the amplification efficiencies from the slope of the line obtained by plotting CT values versus the logarithm of relative cDNA concentrations.
Purity and homogeneity of the retinoid receptors were verified by mass spectrometry analysis in denaturing conditions: proteins were diluted to 5 pmol/µl in a 1∶1 water-acetonitrile mixture (v/v) acidified with 1% formic acid.
Mn-doped ZnO has also aroused lots of interest because it had been predicted to be a room-temperature diluted magnetic semiconductor [9], which was later verified by experiments.
All samples were diluted to 10 nM in RNase free water, verified by Qubit 2.0 flourometer (Invitrogen).
The determination of constituent concentrations in dilute acid hydrolysate and enzymatic hydrolysates were verified by HPLC.
The transcription of a selection of genes was verified by Northern hybridization and primers used for probe amplification are listed in Table 4. Overnight cultures were diluted 100-fold in LB broth and incubated for 2 h.
The overnight solution was diluted to the desired concentration with sterile phosphate buffered saline (PBS) and the concentration of viable bacteria was verified by quantitative plate counts in duplicates.
And it has been verified by Jim Romenesko.
Fox's call was verified by history.
Compliance is verified by an outside auditor.
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