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All mutations were verified by automatic sequencing (AGOWA, Germany).
All reported assignments were verified by automatic and manual interpretation of spectra from Mascot and Proteinlynx search engine and MODi in a blind mode [37], [38].
The nucleotide sequences of inserts were verified by automatic sequencing.
The sequences of all plasmids were verified by Automatic sequencing (Macrogen USA, Rockville, MD, USA).
The sequences of pDCW174 and pDCW175 were verified by Automatic sequencing (Macrogen USA, Maryland).
DNA sequences of BTG2/TIS21 in pcDNA3-HA were verified by automatic sequence analysis (ABI 377).
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In Section 3.1, we make a more careful analysis of T VP, to determine if any marks which can be verified by an automatic system are not passed either by EVA or experts: the worst case scenario.
24 Positive mutations were then verified by direct automatic sequencing.
Quality of data was verified by the automatic artefact recognition of the NIRO 300 Spectrometer, continuous evaluation of the measured parameters and continuous visual observation of the measured curve for artefacts.
All constructs were verified by automated sequencing.
Also, the ontology for RADL is verified by OntoCheck in automatic mode and evaluated by a metric-based approach in manual mode.
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