Exact(18)
The quality and yield of total RNA were verified by assessing the integrity of 28S and 18S rRNA by formamide-based denaturing agarose gel electrophoresis (Masek et al. 2005) and by 260/280 nm ratio spectrophotometric assessment.
Functional homology was verified by assessing the transcriptional response of the genes and up-regulation of corresponding proteins after heat shock stimulus.
Fraction purity was verified by assessing the presence of specific markers for epithelium and mesenchyme, E-cadherin and vimentin respectively.
The proportional hazards assumption was verified by assessing the parallel nature of curves in log-log plots.
Included studies were screened by title and abstract and the full paper verified by assessing the contents of the paper based on the set criteria.
The intraobserver variability of LvV and LvA calculations was verified by assessing twice the LvV and LvA for 12 random patients included in our population.
Similar(42)
Efficiency of all inhibitors was verified by WB by assessing the phosphorylation level of the kinase itself or a known downstream substrate unrelated to NFs (data not shown).
In combination with these data, we performed ChIP assays on κB sites in 14 of the κB -upregulated genes (verified by qPCR) to assess whether they were direct targets of p50 and Bcl-3.
These events were always verified by researchers, who directly assessed the reports of adverse reactions during scheduled clinical visits, reporting comments on clinical files.
Appropriate fractionation was verified in all experiments by assessing the enrichment of acetylated histone H4 in the chromatin fraction and the enrichment of Carboxypeptidase Y (CPY) in the soluble fraction.
The documents, originally given to Riggs by the Chilean defense ministry, were verified by a senior Riggs executive assessing the sources of the general's wealth.
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