Sentence examples for verified by amplifying from inspiring English sources

The transformants were verified by PCR to determine if integration of the vector construct had occurred, followed by analysis of expression from cDNA (Fig. 2).The quality of the synthesized cDNA of the transformants was verified by amplifying a short terminal fragment of the beta- actin gene as control.

Quality of the modified hexadecyltrimethylammonium bromide (CTAB) DNA extractions was verified by amplifying a tick 16S mtDNA fragment (12).

Correct upstream and downstream integration was verified by amplifying across both junctions by colony PCR using primer pairs oLC2802/oLC275 and oLC2804/oLC300, respectively.

The precise cassette exchange in both lines was then verified by amplifying and sequencing the complete PUb-DsRed marker cassette flanked by the heterospecific lox-sites.

BM engraftment was verified by amplifying the male-specific Sry gene from the peripheral blood of recipients using the primers 5'-CAGCTAACACTGATCTTTC-3' and 5'-TTACTGGCCAGAAT-3' [ 9].

No SNPs were observed and the putative RNA editing sites detected in Tcasα6 and Tcasβ1 were verified by amplifying and sequencing genomic DNA present in the extracted total RNA, which was first treated with DNase-free RNase (Roche), using primers recognising intron DNA [see Additional file 4 for primers used].

In brief, replicate PCRs were performed amplifying 100 bp to 200 bp around the coding SNP of interest; products were verified by agarose gel electrophoresis and sequenced using ABI Big Dye chemistry and capillary electrophoresis on an ABI 3730 sequencer (Applied Biosystems).

Fox's call was verified by history.

And it has been verified by Jim Romenesko.

Compliance is verified by an outside auditor.

Results to be verified by agents".