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Resulting mRNA was quality verified as above and combined in equal molar ratios across tissues.
Open pollination of the second F1 (1854) produced 30 progeny plants (verified as above), three of which survived to flowering.
Dried crude foldamer was purified by preparative reverse-phase HPLC, dried by lyophilization and mass and purity was verified as above.
Further details of the process are given in Additional file 5. We identified the protein sequences of 1,725 genes differentially expressed in C. elegans developmental stages [ 61] and selected, using our OrthoMCL clustering, those apparently lacking orthologues in R. culicivorax and T. spiralis (verified as above).
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Other cases are easily verified as the above arguments.
E. coli strain HT115 was thus transformed with plasmid construct L4440-KERP1 that was designed for the expression of dsRNA targeting E. histolytica virulence factor KERP1 [12] and dsRNA expression was verified as described above (Fig. 1).
The presence of the transgene was verified as described above.
PCR products amplified were verified as described above.
The integrity and quantity of the amplified RNA were verified as described above.
Sensitivity of gene deletion strains specific to the Gcn5 HAT complex was verified as described above.
RNA was extracted from the sample biological sample as for hybridization of the Vitis Genome Array and its integrity was verified as described above.
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