Sentence examples for verified and subsequently from inspiring English sources

Full-length Pdu-clock, Pdu-bmal and Pdu-6-4photolyase sequences were PCR amplified from cDNA, subcloned into pJet2.1, sequence verified and subsequently subcloned into pAC5.1/V5-HisA, generating pAct- Pdu-clock, pAct- Pdu-bmal and pAct- Pdu-6-4photolyase.

Three individual fingers (F1, F2 and F3) were amplified using primers specific to their desired backbone position, followed by an overlapping PCR step to place them together into a single ZFP fragment which was cloned, sequence verified and subsequently subcloned in frame with a FokI nuclease variant in a pCS2 expression plasmid.

Thus, an effective numerical scheme is developed, verified and subsequently used for extensive parametric studies.

Each insert was sequence-verified and subsequently transferred into Gateway destination vector pDEST47 (mammalian expression vector, pCMV promoter, ampicillin resistant) (Addgene) or bacterial expression vector pDSW206-ccdB-FLAG.

Each insert was sequence-verified and subsequently transferred into Gateway destination vectors pDSW206 (bacterial expression vector, ColE1 ori, ampicillin resistant), containing the MyoD open reading frame, or into the low-copy gateway destination vector, pNG162-ccdB-TEM-1 (spectinomycin resistant).

This allows to pool risks and uncertainties, and subsequently, to resell these verified credits to the carbon market or use them for national compliance purposes.

Figure 6 shows these samples (blue dots) scattered about the verified global maximum likelihood estimate (MLE) of the triplet obtained in [ 5, 26] and subsequently confirmed algebraically in [ 23].

And these three designations are verified and monitored by animal rights organizations.

1,461 3′UTR clones successfully sequence verified and passed this step.

CFA verified and confirmed the presence of the six factors.

Twenty-four of 40 (60%) cases were categorised as "verified" and 16 as "unverified".