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In agreement with the initial study of Swinnen et al. [ 3] we identified the experimentally verified QTL1 located on chromosome V between coordinates 116,000 and 117,000, containing the causative gene URA3.

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We verified three common QTL located in the same or similar chromosome regions, one for ILAU at all positions in bin 5.06, one for three measured traits in bin 9.06, and one for ILSec in bin 10.04 across two RIL populations (Table 5).

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1,461 3′UTR clones successfully sequence verified and passed this step.

CFA verified and confirmed the presence of the six factors.

In the case of cg0771, cg0922 and cg0527 the verified DtxR binding sites were located within the deduced promoter region, which is consistent with the physiological role of the DtxR protein as transcriptional repressor of gene expression.

Northern analysis of sCAC381 revealed two bands (~300 bp and ~174 bp), indicating possible RNA processing or two transcriptional start sites (TSS for the larger transcript may be located upstream of the regular TSS, but this needs to verified using either strand specific sequencing or 5'RACE).

Gene variants were synthesized, sequence-verified and cloned under control of the T7 promoter (Materials & Methods).