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We verified abundance of transcripts in biological replicates with real time RT- PCR (RT-qPCR) using random hexamers.
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To verify the abundance of each protein, we have performed a bio-informatic analysis using ImageMaster software with 5 gels of each cell line.
To verify transcript abundance we used fluorogenic PCR probe designed to anneal to exon 2. Grm7 expression measured by qPCR showed significantly higher level in 129P3/J mice versus DBA/2J and SWR/J strains (p < 0.01).
Ten of these genes were selected for further quantitative RT-PCR (qRT-PCR) analysis to verify the abundance of mRNA present in the original totRNA samples from the six Holstein-Friesians used to generate the aRNA for the microarray experiment (Table 8).
To verify transcript abundance estimates for these genes, and extend the analysis to two additional HPI axis genes (crhr1, pomc), quantitative real-time reverse transcription PCR (qPCR) was used to examine mRNA expression across a broader range of time points (28 - 98 dph; Figure 6).
In order to detect and verify the abundance of sequences similar to TIR-220 and TIR-FD, genomic DNA of D. virilis and D. americana was dot-blotted onto a positively charged nylon membrane (Roche) and hybridized with cloned TIR-220 and TIR-FD probes separately.
Subsequently, we verified experimentally the abundance of antiparallel β-pleated sheet in the structure of cuticle proteins (Iconomidou et al., Insect Biochem. Mol. Biol. 31 (2001) 877).
Consequently, we have also verified experimentally the abundance of antiparallel β-pleated sheet in the structure of cuticle proteins (Iconomidou et al., Insect Biochem. Mol. Biol. 31 (2001) 877).
We also verified a higher abundance of transcripts related to respiration (2.96%) in comparison with the ones involved in the fermentative metabolism (0.64%).
It is important to note that quantitative PCR analysis verified that the abundances of Bacteroides sp., Neisseria sp., and Actinomycetes sp. were high compared with that of Streptococcus sp. in group CI versus groups CC and HC.
Alteration in transcript abundance was verified for four of these ORFs by real-time qRT-PCR (Table 2).
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