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For each precomputed model, PMP displays technical information such as the date of creation and date of verification, the sequence identity of the template and the expected model accuracy based on the evolutionary distance between the target and the template.
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For verification of the sequence of targeted sites, PCR products were ligated into pBLUE-T vectors and sequenced.
The selected clones after verification of the sequence were subjected to expression studies.
After verification of the sequence, the trxA gene was excised from this plasmid with NdeI and XhoI, and ligated into the same restriction sites of pET26b (Novagen, Inc ., downstream of the T7 promoter and upstream of an in-frame His(6 tag sequence.
After verification of the sequence, the PCR product was cloned into the modified pCAMBIA1305.
After verification of the sequence the fragment was introduced into the pTRV2-attR1-attR2 vector [ 43] via LR recombination.
Selected cloned products were sequenced (Genepool, University of Edinburgh, UK) to permit verification of the sequence of the amplified TRA/TRD chains.
After verification of the sequence integrity, pCAMBiA vectors carrying the CYP79A61 or eGFP construct and the construct pBIN::p19 were separately transferred into Agrobacterium tumefaciens strain LBA4404.
After verification of the sequence, the two inserted restriction sites were used to clone the fragment into the expression vector pHIL-S1 resulting in pEng2.
Both were ligated into the pGEM-T vector (Promega, Mannheim) and after verification of the sequence, transferred into the pBluescript II SK (Stratagene, Amsterdam) vector.
All three fragments were ligated into the pGEM-T vector (Promega, Mannheim) and after verification of the sequence, transferred into the pBluescript II SK (Stratagene, Amsterdam) vector using different restrictions sides inserted by the primers leading to a precursor construct.
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