However, in the verification test, the expression rate of YER034W showed the lowest correlation among the three genes.
Fig. 4 Real-time PCR verification of the expression of OsRUS genes in tissues at vegetative and reproductive stages.
For the verification of the expression of the pca gene clusters, the transcripts of the pca genes in the strains under no H2O2-stress conditions were analyzed by RT-qPCR (Table 1).
Expression array experiments were performed using the Affymetrix Human Genome U133 Plus 2.0 Chip (Affymetrix, Santa Clara, CA, USA) that encodes 54,000 probe sets (with 47,000 transcripts and variants that includes 38,500 well-characterized genes) for integrative expression analyses of RNA mapping and histone acetylation, and for verification of the expression of novel transcripts.
Verification of the expression levels of genes was performed by quantitative RT-PCR using EvaGreen (Botium, Hayward, CA, USA).
The leads provided by the present GWAS, and general verification by the expression analyses comparing phenotypic extremes, now require a systematic ontogenic analysis from before hatching, where feasible.
Here, we have tested these possibilities using oligonucleotide microarrays with verification of the expression of selected genes by real time PCR (qPCR) and Western blotting.
Independent verification of the expression profiles predicted by microarray was conducted using the same pool of RNA with qRT-PCR and semi-RT-PCR.
Accordingly, we expected that detail verification of the expression patterns of OMPs could be a good example to understand the fate of 3R-derived paralogs.
Independent experimental verification of the expression levels obtained in our transcriptome and translatome experiments was performed for a set of differentially expressed T. cruzi genes.
