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A verification sample set consisting of 25% of all samples in the study was randomly selected and the identification of this set was blinded.
For the verification sample set, a unique blinding key was generated and provided exclusively to a third party reader (K.C ., unaffiliated with the study centers or SomaLogic, to score and report the final verification results.
As such, the first verification sample comparing paper listings to the electronic BCG Registry comprised 4,987 records.
With respect to data quality ascertainment in the second verification sample, 4,709 (94.7%) out of 4,972 vaccination certificates were found in the electronic BCG Registry.
Following institutional approval, each ICU in the verification sample provided a list of all blood cultures (BCs) performed over 3 months, and the case records of 5 20 patients with positive BCs.
We performed the preparation of standard solutions, quality control (QC) verification, sample preparation, and instrumental analyses as previously described (Calafat et al. 2004) with slight modification of the sample preparation as described herein.
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Calibration verification samples were analyzed prior to analyzing samples.
Verification samples are presented, and finally a practical application is analyzed where results are compared against those obtained from the finite element method.
From both the training/test samples and the verification samples, there is a notably high correlation between the outcomes of ANN and IP and the actual results, which varies between 97.18% and 99.87%.
Samples for both spore and ATP assays were collected prior to critical spacecraft assembly events (e.g., environmental thermal vacuum, acoustic, and vibrational testing; transportation of the spacecraft from the primary assembly clean room; hardware rework; hardware last access; and NASA Planetary Protection Officer verification sampling).
For rHBD1 identification and sequence verification, samples from selected FPLC fractions were separated using 12% SDS-PAGE gel.
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