Using the resulting regression equations to predict dimensions for the 25 verification pools yields average residuals near zero, although moderate variations do exist.
In addition, if the markers used have been mapped on a genetic map, mapping data can be used for the verification of pool deconvolution.
Upon pooling, manual verification and inspection of truncated hits, 618 NBS-LRR genes were identified in H. brasiliensis.
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Total nucleic acid extraction was performed followed by miRNA expression profiling of 378 miRNAs interrogated using TaqMan MicroRNA Arrays Pool A and verification of candidate miRNAs.
ROC curves were calculated for the discovery (n = 47), verification (n = 78), and pooled (n = 125) sample sets.
This new implementation provides a high-throughput and low cost approach to BAC library screening and deconvolution of clone pools, and tremendously reduces work load otherwise required for PCR screening and verification of a large number of pools [ 6- 9].
The data analysis in this study was initially performed using SPSS 21 with verification of homogeneity of the study pool as suggested by Hedges (1992).
A positive clone in a BAC plate is located by only three types of pools; the other three types of pools are used only for verification.
The library was screened by quantitative PCR (qPCR) through successive steps using BAC super-pools and pools, serial dilutions and clone identify verification by amplicon sequencing.
In addition, there have increasingly been calls to improve the availability of participant-level datasets and statistical code after journal publication to enable verification and replication of analyses, facilitate pooling with other studies, and accelerate research through open knowledge sharing.
