Sentence examples for verification of the expression from inspiring English sources

Fig. 4 Real-time PCR verification of the expression of OsRUS genes in tissues at vegetative and reproductive stages.

For the verification of the expression of the pca gene clusters, the transcripts of the pca genes in the strains under no H2O2-stress conditions were analyzed by RT-qPCR (Table 1).

Expression array experiments were performed using the Affymetrix Human Genome U133 Plus 2.0 Chip (Affymetrix, Santa Clara, CA, USA) that encodes 54,000 probe sets (with 47,000 transcripts and variants that includes 38,500 well-characterized genes) for integrative expression analyses of RNA mapping and histone acetylation, and for verification of the expression of novel transcripts.

Verification of the expression levels of genes was performed by quantitative RT-PCR using EvaGreen (Botium, Hayward, CA, USA).

Here, we have tested these possibilities using oligonucleotide microarrays with verification of the expression of selected genes by real time PCR (qPCR) and Western blotting.

Independent verification of the expression profiles predicted by microarray was conducted using the same pool of RNA with qRT-PCR and semi-RT-PCR.

In addition to the verification of the gene expression associated with T1D, we also identified genes with significant expression changes in T1D patients with diabetes complications.

Further verification of the protein expression level of S100A2 by Western blot analysis using an antibody specific to S100A2 confirmed that the expression of S100A2 in KKU-M213 is obviously higher than KKU-100 cells), in compliance with the expression profile of proteomic analysis.

L.S. did the verification of the transgene expression and mainly performed the animal experiments.

Additionally, quantitative real-time PCR data provided an independent verification of the microarray expression values.

Verification of the gene expression measured by microarray with Q-PCR.