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Verification of stable reference genes in this strain is essential for qRT-PCR data normalization, although results have been reported for other Alternaria sp. strains.
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Total recovery was checked by visual inspection to ensure complete healing of the surgical incision and by verification of a stable daily pattern of Tb variations.
After verification of a stable evaporation rate for at least five 11-s intervals, droplets are ejected continuously into the weighing vessel for another five intervals.
However, reliable relative quantification can only be performed if an accurate normalization is performed following the choice and verification of suitable (i.e. stable) HKGs.
The experimental verification of this model requires the availability of stable, non-aggregated, colloidal nanoparticle suspensions.
For generation of stable cell lines, N2a cells were transfected with Lipofectamine 2000 and selected in growth media supplemented with 800 µg/mL G418 for at least 2 weeks until the verification of transgene expression.
Verification of protein knockdown (KD) efficiency, and total Cx43-GFP protein levels in stable and transient Cx43-GFP/YFP expressing HeLa cells after BECN1 KD, were assessed by western blotting 72 h post-RNAi transfection.
But there is no verification of this.
Independent verification of the data seemed imminent.
Independent verification of Mr. Baryalai's claims was not possible.
"We had independent verification of that," Dyer said.
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