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Verification of markers.
In addition, several methods of monitoring nuclear reprogramming are presented, including the use of transgenic markers, activation of telomerase as an ES-specific marker, light and electron microscopic observation of structural changes in the nucleus, and the verification of surface marker expression and differentiation potential of stem cells.
BAC FISH to pachytene chromosomes was used for verification of several marker anchor points from the physical map, and for finalizing the ordering of the MTP sequences in the euchromatic regions.
DOI: http://dx.doi.org/10.7554/eLife.07789.006 10.7554/eLiFigure89.007 Figure 1 figure supplement 3. Generation and verification of a marker-free Plasmodium berghei line expressing mCherry under control of the uis4 promoter, using GIMO transfection.
Pre-characterization of the CGR8 cell line was performed in our laboratory, which included the verification of stem cell marker expression and neuronal differentiation.
This leads to the verification of already tested markers rather than testing new promising markers for which no prior knowledge is available [89].
Marker verification A group of markers are verified together for a control transfer instruction.
(c) Verification of the universal markers.
With the possibility of multiplexing using proteomic methods such as MRM-MS, the time required for preclinical verification can be reduced as tens of marker candidate proteins can be monitored concurrently in clinical samples.
Independent verification of these results by phenotypic-marker-based classical mapping has not been performed.
To validate "cancer stem cells" sub-populations, OncoTrack uses well-established assays such as immunocytochemical detection of known stem cell markers and verification of tumorigenic potential by xenotransplantation.
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