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Histological verification of lesion location was performed after behavioral testing.
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Therefore, another reference standard was used: a minimum of six months was used for verification of lesions on follow-up control examinations.
For verification of the lesion status, all staging and follow-up examinations, histopathology of biopsies, and clinical data including the serial follow-up examinations were used.
Histologic verification of all lesions in stage 4 patients with metastases is impossible.
At the last visit, colposcopically directed biopsy samples were taken for histological verification of suspected lesions (=end histology).
An increase in a tumor marker ≥25%% triggered a CT scan for verification of new lesions consistent with PMP relapse.
Briefly, all bone lesions showed osteolysis on CT; all pigs were necropsied for verification of the presence of lesions (both OM and ST).
Even when histological examination of the resected liver is used as the 'gold standard' the verification of very small lesions is questionable since most specimens are sectioned at 1 cm intervals.
Although in common practice it is not feasible to obtain histological verification of all (benign and malignant) lesions, and a reasonable reference standard is provided by follow-up of these lesions, partial verification bias may not be fully excluded.
Since the number of animals in each group was corrected after histological verification of the extent of the lesions, the sample size for each group differed from the initial group size and is reported in the corresponding Results section.
The important role of XPA is thus damage verification to ensure that repair occurs only at the point of lesion [ 9].
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