The Ntapi gAHA1 transgenic plants reported here will provide an important tool for AHA1 isoform-specific biochemical analysis, identification of new protein interactions, and verification of interactions previously identified using heterologous techniques.
Primary curators were junior biologists (MSc students) who were encouraged to use all information resources available to first build up an overall picture of these pathways prior to more detailed analyses and literature-based verification of interactions.
We applied this technique in Sprints 4 and 5, as follows: After the software engineer responsible for the interaction modeling finished the diagram, the reviewer responsible for the verification of the interaction model used the IMID technique with the aid of a defects reporting form.
The number of defects, the inspection time and the indicators of effectiveness and efficiency of each subject (software engineer responsible for the verification of the interaction model used the IMID technique) are described in Table 4 (for Sprint 4 and 5).
The PSI-MI data standard was developed to facilitate the exchange, comparison and verification of molecular interaction data (32, 33).
For example, protein-protein networks obtained from Yeast two hybrid experiment (Y2H) are in vitro verification of physical interaction.
In some circumstances we accepted one piece of published evidence if the paper described extensive experimental verification of the interaction.
For verification of the interaction immunoprecipitation of GFP-Sec7 was repeated and probed for the presence of coronin and subunit α-4 (psmA4, (DDB0214953)) of the 20S proteasome using mAb 176-3-6 and 159-83-10, respectively [ 48, 49].
Dnm1 has recently been identified as a putative LRRK2-interacting protein in a mass spectrometry-based screen of brain synaptosomes, although verification of this interaction was not provided (24).
