This will be a valuable technique for molecular and population genetic studies, as well as for verification of genome-wide data sets.
As a verification of the genome regions identified in this study we compared the genomic locations of previously reported seed protein and oil QTL with the physical positions of the markers showing significant associations in this study.
Reboul, J. et al. C. elegans ORFeome version 1.1: experimental verification of the genome annotation and resource for proteome-scale protein expression.
A list of a forward and reverse primers used in this study for verification of whole genome sequencing.
Additional sampling of plastid genome structures and verification of previously published genomes will be necessary to more fully examine this issue.
Although such sequences do not currently contribute to gene discovery, they will be valuable for the verification of gene models or transcribed regions in the completed genome sequence.
This approach is ideal for draft phase genomes and ongoing annotation efforts in completed genomes where the emphasis lies on the establishment and verification of gene annotation and an initial assessment of metabolic capabilities.
To support gene discovery and gene model verification in the genome of the wheat leaf rust fungus, Puccinia triticina (Pt), we have generated Expressed Sequence Tags (ESTs) by sampling several life cycle stages.
Indeed, full-length cDNA clones from many species from Drosophila to human have been collected and characterized [ 12- 16], providing important information for verification of genes in a genome and for studying gene functions.
Ultimate verification of actual gene number and genome duplication as a potential causative mechanism would require sequencing of one or more dinoflagellate genomes, which will also further validate the eukaryotic log gene number-log genome size correlation empirically derived in this study.
After verification of their deletions from the genome (Fig. S3A), we first tested the effects of deleting these gene clusters on the growth of G. sulfurreducens PCA with fumarate as the terminal electron acceptor.
