Sentence examples for verification of expression from inspiring English sources

Even though RT-qPCR is a powerful tool to achieve this goal, a systematic verification of expression stability for reference genes used for RT-qPCR data normalization is still absent in Drosophila.

Verification of expression of recombinant products was performed by total crude protein resolution on SDS-PAGE followed by Western blot analysis using a His-tagged Monoclonal antibody (EMD Biosciences, La Jolla, CA).

Seven candidate miRNAs were chosen for verification of expression using stem-loop RT-PCR.

It was beyond the scope of our analysis to perform such a verification of expression data.

Verification of expression profiles of the Illumina sequencing data was carried out by qRT-PCR analysis.

Ten genes were chosen for verification of expression patterns by quantitative real competitive PCR and real-time PCR.

Based on qPCR verification, trends of expression differences of some above genes agreed with those obtained by Illumina sequencing (Table 2).

Real-time PCR technology has removed many of the difficulties associated with quantitative gene expression studies [ 1], and real-time quantitative RT-PCR (qRT-PCR) is rapidly being adopted as a standard method for in-depth expression studies, including studies of alternative splicing, verification of microarray expression results, and molecular diagnostics [ 2- 5].

Expression array experiments were performed using the Affymetrix Human Genome U133 Plus 2.0 Chip (Affymetrix, Santa Clara, CA, USA) that encodes 54,000 probe sets (with 47,000 transcripts and variants that includes 38,500 well-characterized genes) for integrative expression analyses of RNA mapping and histone acetylation, and for verification of the expression of novel transcripts.

Verification of protein expression by WB confirmed that p85β, p55γ and p110α are indeed predominantly expressed, and that p85α and p110β are effectively weakly expressed (Fig.  1a, right side of panel; 1b, filled columns).

The appropriate plasmid clones were selected after sequencing and verification of protein expression by Western blot.