Thus verification of in vitro findings in experimental animals in vivo is advisable to overcome limitations of the in vitro system.
In general, genes associated with drug response and safety require further validation and verification, including in vitro and in vivo functional studies, animal studies, and large randomized controlled trials (Reynolds, 2012).
Model verification of the in vitro data indicated that, among the cytokines elevated in patients, IL-6 is the key contributor to suppression of CYP450 activities.
In the model, we have indicated that both Irga6 and its GMS attenuator are in the GDP-bound state but this view, though plausible, needs verification by an in vitro reconstruction of the interaction since Taylor and colleagues reported some years ago that the guanine nucleotides co-precipitated with Irgm3 from IFNγ-induced cells were 96% GTP to 4% GDP (Taylor et al, 1997).
In this work, a prototype nitinol foam cage (NFC) incorporating SMP foams has been designed and evaluated in multiple mechanical and in vitro verification tests.
d In vitro verification of size effect on endocytosis tested after 1 h co-incubation (blank columns) and after 4 h co-incubation (grey columns) with Au/PAMAM concentration at 10−7 μg/cell.
For the in vitro verification of the size effect on endocytosis tested after 1 or 4 h co-incubation, a more obvious size effect-induced difference on cellular uptake was detected that Au/PAMAM with small sizes tended to uptake more than others (Fig. 3d).
Verification of the mechanism in vitro may be complicated by the requirement for additional cofactors present in vivo and a potentially very low rate constant.
In this paper we have used a potent combination of in silico prediction and in vitro verification to characterise the peptide binding specificity of the previously poorly- characterised human MHC molecule HLA-Cw*0102 HLA-Cw*0102
For example, protein-protein networks obtained from Yeast two hybrid experiment (Y2H) are in vitro verification of physical interaction.
