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For verification assays, transduced hMSCs were seeded in 96-well plates.
Four of the 8 candidates (TRAF3IP3, ING4, ZNF131, and PHF7) were validated as ERα pathway repressors in the triplicate verification assays (Fig. 1B); their bioinformatics analyses are shown in Table 1.
In this study, we employed Solexa sequencing of pooled sera samples followed by multiple RT-qPCR verification assays of individual samples to generate disease-associated serum miRNA profiles.
Taqman verification assays were analysed by first normalising the CT of a selected assay to the C. elegans miR-39 Taqman CT for the same sample.
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As a positive control for the depletion verification assay, and to enumerate cultivable microbes with the fecal collection procedure, fecal pellets from untreated mice were collected with the above described procedure.
Mycoplasma assays, verification of morphology and growth curve analyses were performed routinely for all cell lines.
Mycoplasma test assays, verification of morphology and growth curve analysis were performed as a routine protocol for all of them.
Significance analysis of microarrays, qPCR verification, drug induction/inhibition assays, and metabonomics indicated that alpha-2u globulin (extracellular region) -socs2 (-SH2-containing signals/receptor tyrosine kinases) -ppp2r2a/pik3c3 (MAPK signaling) -hsd3b5/cav2 (metabolism/organization) plays a vital role in early development.
Hence, STEP3 is useful for the verification of the STEP2 assays, yet descriptive but often not necessary for the assessment of VUS pathogenicity.
Using an online database and with verification from T2 binding assays, the first two HLA-A*02 01-restricted HLA-A*02 01-restricted–1211 and S978–986) were identified in tHLA-A*02 01-restrictedoV.
Selected genes were studied in two steps: (1) using TaqMan Low Density Arrays (TLDA) targeting 89 genes on a set of 12 NB tumour samples, and (2) 12 genes were selected from the TLDA analysis for verification using individual TaqMan assays in a new set of 13 NB tumour samples.
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