Indeed, each of our 12 promising candidates requires further verification and validation using quantitative selected reaction monitoring assays on a larger OvCa cohort.
While TOD strategies are emerging cases, they are merely qualitatively discussed in China, lacking practical indicators and quantitative verification for supporting the real urban plan.
To validate the quality of both the used ESTs and our bioinformatic analysis, ten sample genes with identified alternative splicing events were selected for experimental verification by RT-PCR and quantitative real time RT-PCR (Additional file 1: Table S2).
In this study we investigated the profile of proteins that were differentially expressed in the plasma of patients with malignant tumour with Abnormal Savda compared with patients diagnosed with other syndromes in Uighur medicine using iTRAQ proteomics, bioinformatics, and quantitative verification, to elucidate the biological basis of Abnormal Savda and identify potential biomarkers.
This approach to determine salt responsive transcripts, was used in a previous study of salt-treated Arabidopsis and verification by quantitative real-time PCR demonstrated the consistency of the method [ 5].
The viscous fluid flows are usually accompanied by detailed quantitative verification and validation procedures, when validation of inviscid/irrotational-based codes mostly means qualitative comparison to published measured data or semi-empirical calculations.
To obtain insight into the working mechanisms of learning a reflex like the VOR we can benefit substantially from both the qualitative and quantitative experimental verifications and falsifications of our computational predictions.
Although one aim of the present investigation was simply to determine the qualitative tissue-specific expression of the MSTN gene and quantitative conclusion without verification from quantitative PCR might be hazardous, it was clearly evident that LcMSTN-1 transcripts were more highly expressed in barramundi skeletal muscle than in other tissues.
Although further verification with quantitative environmental and economic performance data would in principle be desirable, this would be impractical due to the general lack of monitoring and recordkeeping of environmental data in many SMEs, and would also face the common conceptual, methodological and fundamental challenges for CP quantification.
