The Oxycon Pro was calibrated according to the instruction manual before each test (Oxycon instruction manual ver. 4.5. Erich Jaeger GmbH, Hoechberg, Germany) [ 18].
Ver Der Moolen Agrees In Principle On SEC, NYSE Settlement.
The maximum allowable power in the system was 10 W at DC voltages not exceeding 2,000 V and a current of 0.01 mA (according to instruction of ThermoScientific ver. 1.0).
Sequencing was performed using an Applied Biosystems ABII) BigDye terminator cycle sequencing ver 3.1 kit as per the manufacturer's instructions, except 0.25 µl of BigDye were used instead of 8 µl (Applied BioSystems, Foster City, CA).
The RACE technique was performed with a TaKaRa kit (5′-Full RACE Kit and 3′-Full RACE Core Set Ver. 2.0) according to the manufacturer's instructions.
Reverse transcription and following amplification of PwRn1 transcripts were carried out with the total RNAs and retrotransposon-specific primers, using RNA PCR Kit (AMV) (Ver. 2.1; Takara) under the manufacturer's instructions.
The primer pairs used in quantitative RT-PCR are listed in Table 2. RNA was reverse transcribed into cDNA using the Takara RNA PCR kit (AMV) Ver. 3.0 (Takara, Kyoto, Japan), according to the manufacturer's instructions; cDNA was stored at −20°C until needed.
Percentage DNA methylation was quantified using primers designed with Pyrosequencing™ Assay Design Software Ver 2.0 (Qiagen) and PyroMark™ MD (Qiagen) according to the manufacturer's instructions.
An approximately 300-bp length cDNA library was prepared using a genomic DNA sample prep kit (Illumina, San Diego, CA, USA), and DNA clusters were generated on a slide using a Cluster Generation kit (ver. 2) on an Illumina cluster station (Illumina), according to the manufacturer's instructions.
THE MANOR Marlene Ver Planck.
