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Using an immunogen peptide containing the exposed VELD sequence, we generated a neo-epitope antibody, D225 that specifically recognized N-GFAP but not C-GFAP or intact GFAP.
To generate the amino-terminal caspase-cleavage product (amino acids 1 225, designated N-GFAP), a translational stop codon right after the glutamic acid of the VELD sequence was introduced by site-directed mutagenesis with 5′-GTGGAGCTTGACTAGGCCAAGCCAG-3′ and 5′- CTGGCTTGGCCTAGTCAAGCTCCAC-3′ as forward and reverse primers, respectively.
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Thus, there is a high veld, a middle veld, a low veld, a bush veld, a thorn veld, and a grass veld.
How quickly the heat leaves the day on the veld.
The veld regions support an enormous variety of natural vegetation.
The boundary between these different varieties of veld is frequently vague, and all of them are usually referred to with the general term veld by the local inhabitants.
The soils of the Lowveld are more varied than those of the other veld regions.
"I understand that the public is clamoring for instant solutions," Ms. in 't Veld said.
Modern study of the veld has centred on its economic, ecological, and archaeological assets.
Sophie in 't Veld, a Dutch member of Parliament, said the commission review raised important concerns.
Nature crept back, like the veld grass, to restate her original claim.
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